Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China
نویسندگان
چکیده
منابع مشابه
Development of real-time reverse transcription PCR for detection of Maize chlorotic mottle virus based on a novel molecular marker
Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, a real-time TaqMan RTPCR assay for efficient detection of MCMV was described. A pair of primers amplifying a 131-bp DNA fragment and a TaqMan probe was designed targeting the novel molecular marker based on MCMV genome analysis sequences. The assay designed was hig...
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Coldberg, K.-B.. and Brakke, M. K. 1987. Concentration of maize chlerotic mottie virus increased in mixed infections with maize dwarf mosaic virus, strain B. Phytopathology 77: 162-167. The concentration of rnaize chlorotic mottle virus (MCMV) was up to chlorophyIl and a lower than normal ratio of chloroplast to cytoplasmic 5.4 times higher in plants infected with both MCMV and maize dwarf rRNA...
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Maize chlorotic mottle virus (MCMV) was first reported in maize in China in 2009. In this study we further analyzed the epidemiology of MCMV and corn lethal necrosis disease (CLND) in China. We determined that CLND observed in China was caused by co-infection of MCMV and sugarcane mosaic virus (SCMV). Phylogenetic analysis using four full-length MCMV cDNA sequences obtained in this study and th...
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15 صفحه اولThe complete nucleotide sequence of the maize chlorotic mottle virus genome.
The complete nucleotide sequence of the maize chlorotic mottle virus (MCMV) genome has been determined to be 4437 nucleotides. The viral genome has four long open reading frames (ORFs) which could encode polypeptides of 31.6, 50, 8.9 and 25.1 kd. If the termination codons, for the polypeptides encoded by the 50 and 8.9 kd ORFs are suppressed, readthrough products of 111 and 32.7 kd result. The ...
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ژورنال
عنوان ژورنال: Journal of Zhejiang University SCIENCE B
سال: 2013
ISSN: 1673-1581,1862-1783
DOI: 10.1631/jzus.b1200275